Thermo Scientific™ GlycanPac™ AXH-1 HPLC Columns

Use this glycan analysis column for qualitative, quantitative, and structural characterization of uncharged and charged glycans present in biological molecules.

Use Thermo Scientific™ GlycanPac™ AXH-1 LC Columns for qualitative, quantitative, and structural characterization of uncharged and charged glycans present in biological molecules. This high-performance, silica-based high-performance liquid chromatography (HPLC) column provides simultaneous separation of glycans by charge, size and polarity. Achieve high-resolution and high-throughput analysis with unique selectivity for biologically important glycans, either labeled or native, by LC-fluorescence and LC-mass spectrometry (MS) methods.

The GlycanPac AXH-1 LC column, based on innovative mixed-mode surface chemistry, combines both weak anion-exchange (WAX) and hydrophilic interaction (HILIC) retention mechanisms for optimal selectivity and resolution power. The WAX functionality provides retention and selectivity for negatively charged glycans while the HILIC mode facilitates the separation of glycans of the same charge according to their polarity and size. As a result, the GlycanPac AXH-1 LC column provides unparalleled capabilities for glycan separations.

Unique Glycan Selectivity Based on Charge, Size and Polarity

• Excellent resolution for both native and labeled glycans
• Useful for both high-resolution glycan profile characterization and easy quantification of glycans based on charge
• Compatible with fluorescence and MS detection methods
• High chromatographic efficiency and excellent column stability

GlycanPac AXH-1 LC columns are based on high-purity and spherical silica substrates in both 1.9 and 3μm sizes for UHPLC and standard HPLC applications, respectively. The various dimensions of GlycanPac AXH-1 LC columns meet the needs of your applications with different resolutions, throughput and instrument requirements.

Column Format

Analytical Column

Diameter (Metric)

2.1 mm

Length (Metric)

250 mm

Max. Pressure

15000 psi (1035 bar)

Particle Shape

Spherical silica

Particle Size

1.9 μm

pH

2 to 8

Pore Size

175 Å

Surface Area

220 m²/g

Temperature

60°C

Stationary Phase

Proprietary

Product Line

GlycanPac

Packing Material

Spherical, Fully Porous, Ultrapure Silica

Column Type

Mixed Mode

Phase

HILIC and Ion Exchange

Type

HPLC Column

Technique

Bio-LC

For Use With (Application)

High-Performance Liquid Chromatography (HPLC)

Limited warranty period: Subject to all warranty requirements and exclusions set out in our Terms and Conditions of Sale, this product is warranted from the date we ship the product and for ninety (90) days thereafter. Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.