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Thermo Scientific Chemicals Guanidine hydrochloride, Molecular Biology Grade
Guanidine hydrochloride is used for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Guanidine hydrochloride is used for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase.
Solubility
Freely soluble in water
Notes
Protect from heat. Store away from oxidizing agents.
Specifications
Melting Point
178°C to 185°C
Color
White
Density
1.34
Quantity
100 g
Beilstein
3591990
Sensitivity
Hygroscopic
Merck Index
144562
Solubility Information
Freely soluble in water
Formula Weight
95.53
Grade
Molecular Biology
Hazard Category
Serious eye damage/eye irritation (category 2), Skin corrosion/irritation (category 2), Acute toxicity (category 4)
Hazard Statement
Causes serious eye irritation, May form combustible dust concentrations in air, Causes skin irritation, Harmful if swallowed or if inhaled
EINECSNumber
200-002-3
RTECSNumber
MF4300000
TSCA
Yes
Recommended Storage
Ambient temperatures
RUO – Research Use Only
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