Thermo Scientific™ Halt™ Phosphatase Inhibitor Single-Use Cocktail

Stabilize serine, threonine and tyrosine phosphorylation of proteins in cells and tissues with this cocktail of phosphatase inhibitors.

Preserve phosphorylation of proteins in cells and tissues with Thermo Scientific™ Halt Phosphatase Inhibitor Cocktail.

• Halt Phosphatase Inhibitor Cocktail protects phosphoproteins from serine/threonine phosphatases, as well as protein tyrosine phosphatases (PTPs) during cell lysis
• The cocktail contains a mixture of four inhibitors of broad specificity including sodium fluoride, sodium orthovanadate, sodium pyrophosphate, and beta-glycerophosphate
• Conveniently packaged in a 100X format, the cocktail is stable at 4°C and is suitable for use with any cell or tissue extract
• The cocktail preserves protein phosphorylation in immunoprecipitation and kinase assays
• Phosphorylation and dephosphorylation comprise a molecular on/off switch that regulates many key biological pathways within the cell including signal transduction, cell division, and apoptosis
• To effectively study phosphorylation activation states, one must inactivate phosphatases to prevent their uncontrolled activity upon cell lysis

Additional Features

• Devoid of protease inhibitors – To protect against both proteases and phosphatases, use Halt Protease and Phosphatase Inhibitor Cocktail (78420)
• Dialyzable – Either dialyze or desalt sample to effectively remove inhibitors from cell extracts before performing procedures that are discovered to be incompatible with cocktail components
• Supplied as a 100X concentrate – effective when used at a final concentration of 1X; however, samples that contain particularly high levels of phosphatase activity may require a final concentration of 2-3X
• Compatibility – compatible with most detergent-based cell lysis reagents, including all Thermo Scientific Pierce Cell Lysis Solutions
• All-in-one format – inhibits both serine-threonine phosphatases and protein tyrosine phosphatases
• Compatible with popular protein assays – works with standard protein assays, including BCA and coomassie (Bradford) assays
• Convenient storage – refrigerator storage (2 to 8°C) saves valuable space in the freezer and allows for immediate use

Product Type

Phosphatase Inhibitor Cocktail

Content And Storage

Upon receipt store product at 4°C. Do not freeze.

Form

Liquid

Product Line

Halt™

Reagent Type

Phosphatase Inhibitor

Inhibitors

Phosphatase Inhibitor

Quantity

1 mL

For Research Use Only. Not for use in diagnostic procedures.