Revvity Health Sciences Inc DepleteX High Expressing RNA Depletion Kit

Supplier:  Revvity Health Sciences Inc NOVA512860

Improve detection sensitivity in fibroblasts and whole blood.While resequencing highly expressed transcripts contributes minimally to biologically relevant information, these transcripts dominate sequencing coverage, hindering detection of low-expressing genes, rare variants, and unique fusion events associated with diseases.DepleteX utilizes Cas9 and a guide RNA set to selectively remove 70% of the most highly expressed transcripts from poly-A selected RNA-Seq libraries obtained from fibroblasts and whole blood. This design optimizes the reallocation of sequencing reads, enhancing coverage of rare, low-expressing transcripts and unique fusion events.The DepleteX High Expressing RNA Depletion kit is used during the RNA-seq library prep workflow starting with fibroblasts and whole blood samples after the ligation step and before the PCR step. This kit is compatible with most RNA-seq workflows; however, we recommend using it with the NEXTFLEX Rapid Directional RNA-seq kit 2.0.

Catalog No. NC3241879