Cytiva His Mag Sepharose™ excel Chromatography Media

His Mag Sepharose excel magnetic beads are designed for small-scale purification and screening of histidine-tagged proteins secreted in eukaryotic cell culture.

Samples that usually cause stripping of metal ions can therefore be added to the medium with retained protein-binding capacity and without pretreatment.

• His Mag Sepharose excel is a magnetic IMAC medium precharged with nickel ions that are very strongly bound to a chelating ligand.
• The medium enables an efficient and simplified workflow for capture, purification, and reproducible screening of histidine-tagged proteins.
• Simplify small-scale purification and expression screening of histidine-tagged proteins secreted in eukaryotic cell culture.
• Purify histidine-tagged proteins directly from samples that contain metal ion-stripping agents and save time and effort.
• Increase target protein yield and decrease degradation through reduced and simplified sample handling.
• Screen histidine-tagged proteins in parallel with high reproducibility and reliable results.
• Capture histidine-tagged protein from different sample volumes; from low to high milliliter scale.
• Simplify handling using visible and dense Sepharose-based magnetic beads.
• Proteins are expressed and secreted into cell culture supernatants from eukaryotic cells such as insect cells or Chinese Hamster Ovary (CHO) cells.
• The high density of the beads allows rapid capture by magnetic devices while the visibility of the beads ensures reliable collection of the bound histidine-tagged proteins during the purification procedure.
• The base matrix is based on Sepharose medium with magnetite incorporated.
• His Mag Sepharose is available as 10% medium slurry in three different pack sizes (2 x 1ml, 5 x 1ml, and 10 x 1ml) and can be used together with different test tubes and magnetic racks.

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pH Range

2 to 12

Support Type

Highly cross-linked spherical Agarose including Magnetite

Content And Storage

4°C to 30°C

Particle Size

63 μm

Quantity

10 x 1 mL

For Use With (Application)

Designed to simplify small-scale purification and screening of His-tagged Proteins secreted in eukaryotic cell culture, used for functional and structural studies research in academia, institutes and discovery Pharma

Type

Chromatography Media