Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Cytiva HisPrep Column

Catalog No. 45002073 Shop All Cytiva Products
Click to view available options
:

Optimize purification of histidine-tagged recombinant proteins. Cytiva Life Sciences™ HisPrep Column is a ready to use column offering convenient scale-up purification of histidine-tagged proteins.

  • Optimized for convenient scale-up purification of histidine-tagged proteins
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
  • Negligible leakage of Ni2+
  • Bed Volume 20ml
  • High binding capacity
  • Convenient and time-saving prepacked HiPrep format with precharged Ni Sepharose 6 Fast Flow medium
  • Media:
  • BioProcess media
  • 90μm average particle size
  • Metrix 6% cross-linked agarose
  • pH stability working range 3-121
  • Metal ion capacity ∽15μmol Ni2+™/ml medium
  • Three years shelf life
  • Stable in commonly used aqueous buffers - 0.1 M HCl, 0.1 M NaOH, 8 M urea3
  • Column:
  • Inside Diameter is 16mm
  • Material of Column hardware and tube is Polypropylene and bed support is Polymethyl-Methacrylate
  • Bed support Filter

  • Bed Dimensions 16 x 100mm
  • Flow rate <10ml/min1
  • Flow Velocity <300cm/h
  • Pressure Max. 1.5 bar

Specifications

Diameter (English) Inner Column

0.6 in.

Flow Rate

<10 mL/min.

Content And Storage

4°C to 30°C

Diameter (Metric) Inner Column

1.6 cm

Volume (Metric)

20 mL

Quantity

Each

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.