Cytiva HiTrap™ HIC Sepharose™ Selection Kit

Use for laboratory and intermediate process-scale separations and final-step purifications. Cytiva Life Sciences™ HiTrap™ HIC Sepharose™ Selection Kit takes full advantage of hydrophobic interaction chromatography providing highest resolution.

• Includes seven 1mL HIC columns, each packed with a different medium for small-scale media screening and selection
• Flexible design for use with syringes, single laboratory pump or chromatography system
• High flowrates and high capacities

Media in Kit Columns:
Phenyl Sepharose High Performance

• For laboratory and intermediate process-scale separations and final-step purifications needing a high resolution

Phenyl Sepharose 6 Fast Flow™ (low sub) and Phenyl Sepharose 6 Fast Flow (high sub)

For initial and intermediate step purifications requiring a matrix with low to medium hydrophobicity

Two available degrees of substitution increases likelihood of finding the optimal selectivity andcapacity for a given application

Butyl Sepharose High Performance

• For intermediate and final purification steps
• Relatively high ligand density enables high dynamic binding capacity for proteins, even at low salt concentrations

Butyl Sepharose 4 Fast Flow

• For initial and intermediate step purifications requiring a matrix with low to medium hydrophobicity
• Often works well even in relatively low salt concentrations

Butyl-S Sepharose 6 Fast Flow

• Least hydrophobic option in the Fast Flow series
• Suited for binding and elution of relatively strong hydrophobic proteins

Octyl Sepharose 4 Fast Flow

• Different hydrophobic character from the phenyl and butyl ligands

Bed Volume

1 mL

Diameter (English) Inner

0.275 in.

Diameter (Metric) Inner

7 mm

Product Type

HiTrap HIC Selection Kit

Flow Rate

< 4 mL/min

Quantity

7 x 1 mL

Includes

Seven HiTrap 1 mL column containing the following media: Phenyl Sepharose high performance, Butyl Sepharose high performance, Phenyl Sepharose 6 fast flow (low sub), Phenyl Sepharose 6 fast flow (high sub), Butyl-S Sepharose 6 fast flow, Butyl Sepharose 4

Material

Polypropylene

Max. Pressure

5 bar [0.5 MPa] (70 psi)

Storage Requirements

4°C to 30°C, 20% Ethanol