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Cytiva HiTrap™ NHS-Activated HP Columns

Catalog No. 45000137 Shop All Cytiva Products
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Diameter (Metric) Inner:
This item is not returnable. View return policy
This item is not returnable. View return policy

Includes

Complete instructions for use and method optimization, plus a variety of connectors for syringe, peristaltic pump, and chromatography systems

Couple ligands containing primary amines easily. Cytiva Life Sciences™ HiTrap™ NHS-Activated HP Columns are prepacked with NHS activated Sepharose™ High Performance.

Prepacked with NHS-activated Sepharose™ High Performance Media for coupling ligand via primary amines at close to neutral pH

  • Designed for use with a syringe, peristaltic pump, or chromatography system
  • Degree of substitution: 10 μmol NHS/mL medium
  • Delivered in 100% isopropanol to protect the active groups
  • Stable in the pH range 3 to 12, depending upon ligand
  • Average particle size: 34μm

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Specifications

Quantity

5 x 1mL

Flow Rate

4 mL/min.

Particle Size

34 μm

For Use With (Equipment)

Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system

Bed Volume

1mL

Product Type

NHS-Activated HP Column

Diameter (Metric) Inner

0.7cm

For Use With (Application)

Preparative affinity chromato graphy, easy coupling of ligands containing primary Amines

Height (English)

0.98 in.

Height (Metric)

2.5cm

Ligand Type

10μM NHS groups/mL medium

Material

Polypropylene

Max. Pressure

42psi

pH Range

3 to 12

Support Type

6% Highly Cross-linked Agarose

Content And Storage

4° to 8°C

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