Invitrogen™ Human CD63 ELISA Kit

Human CD63 quantitates human CD63 in serum, plasma, supernatant.

Human CD63 quantitates human CD63 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human CD63.

CD63 (LAMP-3, lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. CD63 is also present in the plasma membrane, usually following cell activation. Hence, CD63 has become a widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas. CD63 is expressed on activated platelets, and is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. CD63 is also present in monocytes and macrophages and is weakly expressed on granulocytes, B, and T cells. CD63 is identical to the melanoma-associated antigen which is ME491 and to the platelet antigen PTLGP40. Diseases associated with CD63 dysfunction include melanoma and Hermansky-Pudlak Syndrome.

Accession Number

C75951; CD 63; CD63; CD63 antigen; CD63 antigen (melanoma 1 antigen); Cd63 molecule; CD63 protein; granulophysin; LAMP-3; Lysosomal-associated membrane protein 3; lysosome-associated membrane glycoprotein 3; mast cell antigen AD1; ME491; melanoma 1 antigen; melanoma-associated antigen ME491; melanoma-associated antigen MLA1; MLA1; Ocular melanoma-associated antigen; OMA81H; tetraspanin-30; TSPAN30; Tspan-30

Assay Range

14 to 3,000 pg/mL

Assay Sensitivity

14 pg/mL

Conjugate

Biotin

Product Type

ELISA

Sample Type

Plasma, Serum, Supernatant

For Use With (Equipment)

Colorimetric Microplate Reader

Gene ID (Entrez)

P08962

Gene Symbol

CD63

Interassay CV

<12%

Intraassay CV

<10%

Kit Contents

Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers

Label or Dye

HRP

Quantity

5 x 96 Tests

Regulatory Status

Research Use Only

Sample Volume

Serum, 50 μL; Plasma, 50 μL; Supernatant, 50 μL

Storage Requirements

2°C to 8°C

Target

CD63

Test Time

1 hr 20 min

Total Assay Time

4 hr 45 min