Invitrogen™ IL-6 Human ELISA Kit

Measure levels of human interleukin-6 in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples using 96-well plates.

Assay IL-6 (human interleukin-6) levels in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples with our Thermo Scientific Human IL-6 ELISA Kits.

Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.

Accession Number

P05231

Assay Range

10.24-400 pg/mL

Assay Sensitivity

<1 pg/mL

Description

Human IL-6 ELISA Kit

Conjugate

Biotin

Immunoassay Kit Format

Colorimetric ELISA (TMB substrate) with Plates

Product Type

ELISA Kit

Sample Type

Plasma, Serum, Supernatant, Urine

Specificity

No significant cross-reaction with with human IL-1a, IL-1ß, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-10, IL-12, TNFa, IFNg, IFNa, GM-CSF, or mouse IL-6

Sufficient For

96 microplate wells

Target Species

Human

For Use With (Equipment)

Colorimetric Microplate Reader

Gene ID (Entrez)

3569

Gene Symbol

BSF-2,BSF2,CDF,HGF,HSF,IFN-beta-2,IFNB2,IL-6

Interassay CV

<10%

Intraassay CV

<10%

Kit Contents

Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Streptavidin-HRP Concentrate, 75µL

Label or Dye

HRP

Measurement

Absorbance at 450nm minus absorbance at 550nm

Precision

Intra-assay CV <10%; inter-assay CV <10%

Quantity

96 Tests

Regulatory Status

RUO

Sample Volume

Plasma, 50 μL; Serum, 50 μL; Supernatant, 50 μL; Urine,50 μL

Storage Requirements

2°C to 8°C

Target

Human

Test Time

1 hr. 20 min.

Total Assay Time

3 hr.