Gibco™ Human Melanocyte Growth Supplement (HMGS)

Human Melanocyte Growth Supplement (HMGS)

A sterile, concentrated (100X) solution intended for use with Medium 254 or Medium 254CF to culture human epidermal melanocytes. Each 5 ml bottle of HMGS contains all of the growth factors, hormones, and tissue extracts necessary for the culture of human epidermal melanocytes and is the correct amount of supplement for a 500 ml bottle of basal Medium 254 or Medium 254CF.

When a 500 ml bottle of medium is supplemented with HMGS, the final concentrations of the components in the supplemented medium are:

Bovine pituitary extract (BPE) (0.2% v/v)

Fetal bovine serum (0.5% v/v)

Recombinant human insulin-like growth factor-I (1μg/ml)

Bovine transferrin (5μg/ml)

Basic fibroblast growth factor (3 ng/ml)

Hydrocortisone (0.18μg/ml)

Heparin (3μg/ml)

Phorbol 12-myristate 13-acetate (PMA) (10 ng/ml)

Concentration

100 X

Product Type

Growth Supplement

Sterility

Sterile

Content And Storage

Store in freezer (-5 to -20°C).

Shipping Condition

Dry Ice

Product Line

Cascade Biologics™

Cell Type

Melanocytes

Source

Human

Species

Human

For Research Use Only. Not for use in diagnostic procedures.