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Invitrogen™ I-Block™ Protein-Based Blocking Reagent
Highly purified Casein-based blocking reagent. Thermo Scientific I-Block Protein-Based Blocking Reagent provides superior blocking compared to both dried milk and BSA.
Supplier: Invitrogen™ T2015
Description
- Biotin-free
- Tested in assays using Tropix substrates and alkaline phosphatase conjugates
- Useful as blocking reagent in membrane-based and immunoassay applications
- Suggested working
- concentration is 0.2% (w/v) for detection of nucleic acids on neutral or positively-charged nylon membranes and for immunoassays and protein detection on membranes (nitrocellulose, PVDF, or neutral nylon)
- Concentration of 3% is recommended for protein detection on positively-charged nylon membrane (such as TropilonPlus™ membrane)
Preparation
Prepared in either Tris- or phosphate-buffered saline buffer with heating (40° to 50°C).
Applications
Can be used with Western-Light™, Western-Light Plus™, Western-Star™, ELISA-Light™, Southern-Light™, and Southern-Star™ Detection Systems.
Specifications
| Reagent | |
| I-Block™, NovaBright™ | |
| Blocking Buffers | |
| Room Temperature |
| 30 g | |
| Western-Light™ Detection System, Western-Star™ Detection System, Southern-Star™ Detection System, ELISA-Light™ Detection System, Southern-Light™ Detection System, Western-Light Plus™ Detection System | |
| Store the product at room temperature. |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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