Invitrogen™ mirVana™ miRNA Mimic, miR-1 Positive Control

miRNA mimics are small, chemically modified double-stranded RNA molecules designed to specifically bind to and mimic endogenous miRNA molecules and enable miRNA functional analysis by up-regulation of miRNA activity.

Catalog Number	Mfr. No.	Quantity
44-640-62 Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More	Invitrogen™ 4464062	5 nmol
44-640-63	Invitrogen™ 4464063	20 nmol
44-640-64	Invitrogen™ 4464064	50 nmol
44-640-65	Invitrogen™ 4464065	250 nmol

Description

miRNA mimics are small, chemically modified double-stranded RNA molecules designed to specifically bind to and mimic endogenous miRNA molecules and enable miRNA functional analysis by up-regulation of miRNA activity.

We have now released the second generation of miRNA mimics, mirVana™ miRNA Mimics, which:

• Exhibit maximum and consistent effect in vitro at low concentration
• Offer superior specificity due to unique Star strand modification
• Target the most updated Sanger content
• Can be used in vitro and in vivo, offering you consistency throughout your entire research project
• Are non-toxic

Positive and negative controls are also available for use along with mirVana™ miRNA Mimics in miRNA up-regulation experiments.

mirVana™ miRNA Mimic miR-1 Positive Control

The mirVana™ miRNA Mimic miR-1 Positive Control is designed to mimic mature miR-1 miRNA after delivery to mammalian cells. miR-1 is associated with down-regulation of many genes. In particular, miR-1-mediated down-regulation of Protein Tyrosine Kinase 9 (PTK9) has been shown to occur at the mRNA level. miR-1 Positive Control is intended for use as a positive control for transfection experiments using mirVana™ miRNA Mimics. Monitor activity of miR-1 Positive Control through its gene silencing of PTK9, using real-time reverse transcription-PCR to detect PTK9 mRNA.

First Generation Mimic Controls

Our first-generation miRNA mimics (and controls), Ambion™ Pre-miR™ miRNA Precursors, have lower specificity and potency, plus a lower strand specificity compared to the new mirVana™ miRNA Mimics. These first-generation mimics are recommended for completing ongoing in vitro miRNA studies. For new studies or in vivo studies, we highly recommend our new mirVana™ miRNA Mimics and their corresponding controls.

Specifications

• Content And Storage: Store at or below –20°C. Do not store in a frost-free freezer.
• RNAi Type: miRNA Mimic
• Label or Dye: Unconjugated
• Shipping Condition: Room Temperature

• Control Type: Positive Control
• For Use With (Application): miRNA & Non-Coding RNA Analysis
• Product Type: miRNA Mimic

For Research Use Only. Not for use in diagnostic procedures.