Applied Biosystems™ Power SYBR™ Green RNA-to-C_T™ 1-Step Kit

The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.

Catalog Number	Mfr. No.	No. of Reactions
4391178 Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More	Applied Biosystems™ 4391178
50-591-795 Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More	Applied Biosystems™ 4389986	200 Reactions

Description

The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.

• Get sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction.
• Formulated to ensure maximum sensitivity and reliability
• Reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets

Includes:

• AmpliTaq™ Gold DNA Polymerase UP for hot start qPCR, minimizing non-specific product formation
• ArrayScript™ UP Reverse Transcriptase, an engineered RT that produces high yields of full-length cDNA
• An additive that reduces primer dimer formation, reducing non-specific products that can cause false positive signals
• RNase inhibitor, to prevent degradation of RNA templates
• A proprietary ROX™ dye that serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations for superb precision on Applied Biosystems™ real-time PCR instruments

Specifications

• Detection Method: SYBR
• Polymerase: AmpliTaq Gold DNA Polymerase
• For Use With (Equipment): 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
• Passive Reference Dye: ROX (Pre-mixed)
• Purity or Quality Grade: UP (Ultra Pure)
• Shipping Condition: Wet Ice
• Sample Type: RNA

• PCR Method: 1-Step qRT-PCR
• Reaction Speed: Standard
• Label or Dye: SYBR™ Green
• Product Line: RNA-to-C_T™, SYBR™
• Reverse Transcriptase: ArrayScript™ UP
• For Use With (Application): Real Time PCR (qPCR)

For Research Use Only. Not for use in diagnostic procedures.