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Invitrogen™ eBioscience™ Indo-1 AM Calcium Sensor Dye (UltraPure Grade)
- Unbound Indo-1 has a peak emission at 475nm
- Upon binding calcium, peak emission shifts down to 410nm
- In flow cytometry this can be measured over time and can be represented as a ratio of the two emission wavelengths
- To optimize the ratio between the two emissions, unbound Indo-1 fluorescence should be collected using a filter above 475nm; bound Indo-1 fluorescence should be collected using a filter below 400nm
- Because the emission profile of Indo-1 is broad, multicolor flow analysis using eFluor ™ 450, eVolve™ 605 or eVolve™ 655 is not possible, however fluorochrome conjugated antibodies utilizing the 488nm or 633 nm are quite compatible with Indo-1
- Molecular weight: 1009.91g/mol
- Peak excitation: 346nm
- Peak emission: 475nm
- Reconstitute in high-quality, freshly opened DMSO
- Recommended concentration: 1 mM
- Once reconstituted, store at −20°C with dessicant and use within a short period of time. Avoid freeze-thawing
Specifications
Color
Blue
Detection Method
Fluorescence
For Use With (Application)
Immunohistochemistry (IHC), Flow Cytometry
For Use With (Equipment)
Fluorescence Microscope
Product Type
Dye
Emission
346⁄475
Product Line
eBioscience™
Quantity
20 x 50 μg
For Research Use Only
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