Invitrogen™ Insulin Mouse ELISA Kit

Sandwich ELISA Kit

The Mouse Insulin ELISA quantitates Ms Insulin in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Insulin. Principle of the method The Mouse Insulin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Insulin is a peptide hormone secreted by beta cells of the pancreatic islets. It regulates carbohydrate, protein and lipid metabolism by enhancing membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Insulin is 51-amino acid polypeptide product produced from a precursor peptide, proinsulin. Proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds and one molecule of C-peptide. Insulin deficiency results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of b-cell origin such as insulinoma.

Accession Number

P01325

Assay Range

6.25-400μIU/mL

Assay Sensitivity

5μIU/mL

Calibration Range

5μIU/mL to 400μIU/mL

Description

Insulin ELISA Kit, 96 Assays, 1 Kit, Mouse Species, Hormones and Receptors Protein Family, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of Insulin in Samples

Detectable Analytes

Mouse, human, rat, porcine and bovine insulin

Assay

Solid-phase sandwich ELISA

Conjugate

Biotin

Detection Method

Colorimetric

Product Type

ELISA Kit

Protein Family

Hormones & Receptors

Sample Type

Plasma, Serum, Supernatant

Specificity

This ELISA pair antibody detects mouse, human, rat, porcine and bovine Insulin.

Sufficient For

96 Assays

Target Species

Mouse

For Use With (Application)

ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis

For Use With (Equipment)

Colorimetric Microplate Reader

Gene ID (Entrez)

16333

Gene Symbol

Ins-1,Ins2-rs1

Host Species

Mouse

Interassay CV

<12%

Intraassay CV

<10%

Kit Contents

Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Anti-Rat insulin precoated 96-well strip plate, lyophilized recombinant rat insulin standard, 20X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, TMB substrate, stop solution, contains 0.2M sulfuric acid, assay diluent C, assay dilue

Label or Dye

HRP

Quantity

96 Tests

Regulatory Status

RUO

Research Discipline

Endocrinology

Sample Volume

Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL

Storage Requirements

2°C to 8°C

Synonym

Ins-1, Ins2-rs1

Target

Mouse

Test Time

1 hr. 20 min.

Total Assay Time

4 hr. 45 min.