Invitrogen™ 2-YT Broth

2-YT Broth is a nutrient-rich microbial growth medium used for cultivation of E. coli and growth of hosts for replication of M13 vectors.

Supplier:  Invitrogen™ 22712020

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Catalog No. 22-712-020

Description

2-YT Broth is a nutrient-rich microbial growth medium used for cultivation of E. coli and growth of hosts for replication of M13 vectors. The 2-YT formulation is similar to Lennox LB medium, but contains double the concentration of yeast extract and a 60% higher concentration of peptone. 2-YT medium allows cells to reach higher density and increase recombinant protein production compared to LB media formulations.

2-YT Broth formulation
Formulation per liter: 16 g SELECT Peptone 140 (pancreatic digest of casein), 10 g SELECT Yeast Extract, and 5 g NaCl.
Note: To prepare 1 L of 2-YT medium, weigh 31 g of powder and add distilled water to 1 L. Adjust the pH value to 7.0 with 5 N NaOH and sterilize by autoclaving for 20 minutes at 121°C.

Primary applications
• Growth of hosts for replication of M13 vectors
• Recombinant protein expression
• Achieve higher plasmid DNA yield

Find the media format that fits you needs
We offer a variety of ready-to-use media formulations including LB Broth (Cat. No. 10855001), Terrific Broth (Cat. No. A1374301) and S.O.C Recovery Medium (Cat. No. 15544034).

Order Info

Shipping Condition: Room Temperature

Specifications

• Species: Escherichia coli
• Format: Bottle
• Preparation Method: Autoclave
• Target Organism Class: Coliforms
• Shipping Condition: Room Temperature

• Content And Storage: • 2-YT Broth Powder (500 g)
  
  Store at room temperature.
  
  
• Media Type: 2-YT Broth (Yeast Extract Tryptone)
• Product Type: Dehydrated Culture Media
• Quantity: 500 g

For Research Use Only. Not for use in diagnostic procedures.