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Invitrogen™ Bacterial Alkaline Phosphatase
Removes 3' and 5' phosphates from DNA and RNA
Supplier: Invitrogen™ 18011015
Includes: A vial of 10X dephosphorylation buffer [100mM Tris-HCl (pH 8.0)].
Description
BAP is active at 65°C for at least 1 hr. and is inactivated by phenol extraction
- Source: Purified from E. coli C90
- Performance and Quality Testing: Endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; dephosphorylation efficiency determined
- Unit Definition: One unit hydrolyzes 1nmol of ATP in 30 min. at 37°C
- Unit Reaction Conditions: 10mM Tris-HCl (pH 8.0), 1.5mM [γ-32P]ATP, and enzyme in 50μL for 30 min. at 37°C
Dephosphorylation of 5'-phosphorylated termini of vector DNA to prevent self-ligation; Dephosphorylation of 5' termini of nucleic acids prior to forward reaction with kinase; Cloning; Restriction Enzyme Cloning
Order Info
Shipping Conditions: Wet ice
Specifications
| Bacterial Alkaline Phosphatase is supplied with a vial of 10X dephosphorylation buffer [100 mM Tris-HCl (pH 8.0)]. Store at -20°C. | |
| BAP | |
| Alkaline Phosphatase |
| Wet Ice | |
| 2500 U |
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Product Title
For Research Use Only. Not for use in diagnostic procedures.
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