Invitrogen™ Champion™ pET SUMO Expression System

Includes

The Champion pET SUMO TA Cloning box contains linearized Champion pET SUMO vector, sterile water, dNTPs, 10X PCR Buffer, control template and primers, T4 DNA ligase, 10X ligation buffer, primers for sequencing or PCR screening, and an expression control. The SUMO Protease box contains SUMO Protease and buffers. The TOP10 box contains 21 x 50μL aliquots of chemically competent E. coli, S.O.C. medium, and a control plasmid. The One Shot BL21(DE3) box contains 21 x 50μL aliquots of chemically competent E. coli, S.O.C. medium, and a control plasmid.

Yield high levels of protein expression in E. coli

• Uses small ubiquitin-related modifier (SUMO) fusion, belonging to growing family of ubiquitin-related proteins, to enhance solubility of expressed fusion proteins
• Contrast to ubiquitin, SUMO is involved in stabilization and localization of proteins in vivo
• After expression, 11 kd SUMO moiety can be cleaved by highly specific and active SUMO (ULP-1) protease at carboxyl terminal, producing native protein
• Enhanced solubility with N-terminal SUMO fusion
• Highly efficient cleavage produces native protein of interest with SUMO (ULP-1) protease
• Highly specific cleavage eliminates chance of protein of interest being internally digested, regardless of its amino acid sequence
• Significantly increased stability with SUMO fusion-can be used for small peptide productionT7lac promoter for high-level protein expression
• N-terminal 6xHis tag for protein detection and purification

Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Constitutive or Inducible System

Inducible

Inducing Agent

IPTG

Promoter

T7, lacO

Product Type

Expression System

Selection Agent (Eukaryotic)

None

Content And Storage

The Champion™ pET SUMO Expression System is provided as a complete system. The Champion™ pET SUMO TA Cloning™ box contains linearized Champion™ pET SUMO vector, sterile water, dNTPs, 10X PCR Buffer, control template and primers, T4 DNA ligase, 10X ligation buffer, primers for sequencing or PCR screening, and an expression control. Store at -20°C. The SUMO Protease box contains SUMO Protease and buffers. Store at -80°C. The One Shot™ TOP10 box contains twenty-one 50 μl aliquots of chemically competent E. coli, S.O.C. medium, and a control plasmid. Store at -80°C. The One Shot¤ BL21(DE3) box contains twenty-one 50 μl aliquots of chemically competent E. coli, S.O.C. medium, and a control plasmid. Store at -80°C.

Antibiotic Resistance Bacterial

Kanamycin (KanR)

Bacterial or Yeast Strain

BL21(DE3)

Cleavage

SUMO

Expression System

E. coli

Expression Mechanism

Cell-Based Expression

Protein Tag

His Tag (6x), SUMO Tag

Cloning Method

TOPO

Quantity

20 reactions

Vector

pET

Product Line

Champion™, TA Cloning™

For Research Use Only. Not for use in diagnostic procedures.