Invitrogen™ Dynabeads™ Untouched™ Human T Cells Kit

Use this kit to isolate pure and viable untouched T-cells from PBMC by negative isolation. The kit depletes B cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes.

Supplier:  Invitrogen™ 11344D

Catalog No. 11-344-D

Includes: 10mL Depletion Dynabeads and 2mL Antibody Mix with monoclonal antibodies towards human CD14, CD16 (a and b), CD19, CD36, CD56, CD123 and CD235a (Glycophorin A)

Description

Use this kit to isolate pure and viable untouched T-cells from PBMC by negative isolation. The kit depletes B cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human T-cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free T-cells are truly untouched, and in perfect shape for any functional assay and application.

• High purity, recovery and viability of human untouched T cells
• Easy to use and to scale up
• No columns required

Methodology:
An antibody mix towards the non-T cells is added to the sample and allowed to bind to the cells. Dynabeads™ are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human T-cells can be directly analyzed in a flow cytometer and used in any application.

Note: A negative isolation strategy isolates pure and truly untouched cells. When maximum cell purity is critical, a positive cell isolation strategy is recommended

Starting Sample:
PBMC

Order Info

Shipping Condition: Room Temperature

Specifications

• Cell Type: T Cells (Whole Population)
• Product Type: Cell Isolation Kit
• Isolation Technology: Negative Isolation
• No. of Tests: 100 Isolations
• Product Line: DYNAL™, Dynabeads™, Untouched™
• Sample Type: PBMC
• Starting Material Cell No.: 1 x 10⁷ PBMCs per isolation

• Content And Storage: The Kit Contains: 10 mL Depletion Dynabeads™ and 2 mL Antibody Mix with monoclonal antibodies towards human CD14, CD16 (a and b), CD19, CD36, CD56, CD123 and CD235a (Glycophorin A).
  
  Store at 2°C to 8°C.
• Final Product Type: Cells
• No. of Cells: Processes ∼1 x 10⁹ cells total
• Output Viability: >95%
• Quantity: 1 kit
• Shipping Condition: Room Temperature
• Target Species: Human

For Research Use Only. Not for use in diagnostic procedures.