Invitrogen™ Dynabeads™ Mouse CD43 (Untouched™ B Cells)

Includes

5mL MyOne; Dynabeads- coated with streptavidin and biotinylated rat monoclonal CD43 antibody against mouse T cells, monocytes/macrophages, NK cells, plasma cells, CD5+ B1 cells, platelets, dendritic cells, granulocytes and erythrocytes

Use this kit to isolate pure and viable untouched B cells from mouse lymphoid organs by negative isolation. The kit depletes T cells, monocytes/macrophages, NK cells, plasma cells, CD5+ B1 cells, platelets, dendritic cells, granulocytes and erythrocytes.

Use this kit to isolate pure and viable untouched B cells from mouse lymphoid organs by negative isolation. The kit depletes T cells, monocytes/macrophages, NK cells, plasma cells, CD5+ B1 cells, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated mouse B cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free B cells are truly untouched, and in perfect shape for any functional assay and application.

High purity, recovery and viability of mouse untouched B cells
• Easy to use and to scale up
• No columns required

Methodology
Dynabeads Mouse CD43 (Untouched B Cells) is added directly to the sample and binds the unwanted cells. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched mouse B cells can be directly analyzed in a flow cytometer and used in any application.

Starting Sample
Spleen or lymph nodes

Order Info

Shipping Condition: Room Temperature

Cell Type

B Cells

Content And Storage

The Kit Contains: 5 mL MyOne™ Dynabeads™-coated with streptavidin and biotinylated rat monoclonal CD43 antibodyagainst mouse T cells, monocytes/macrophages, NK cells, plasma cells, CD5+ B1 cells, platelets, dendritic cells, granulocytes and erythrocytes.

Store at 2°C to 8°C.

Product Type

Antibody Coated Bead

Final Product Type

Cells

Format

Bead

Isolation Technology

Negative Isolation

No. of Cells

Processes ∼2 x 10⁹ cells/kit, Processes ∼2 x 10⁹ cells total, Processes ∼1 x 10⁹ cells/kit

No. of Tests

40

Product Line

DYNAL™, Dynabeads™, Untouched™

Purity or Quality Grade

Research Grade

Quantity

1 kit

Reactivity

Mouse

Sample Type

Lymph Node, Tissue Digests, Lymph nodes, Spleen

Shipping Condition

Room Temperature

Starting Material Cell No.

5 x 10⁷ cells/test

Target Species

Mouse

For Research Use Only. Not for use in diagnostic procedures.