Invitrogen™ ElectroMAX™ DH10B Cells

Description

Includes

ElectroMAX DH10B Cells: 5 vials, 100μL each, pUC19 DNA (10pg/μL): 1 vial, 50μL, S.O.C. medium: 2 bottles, 6mL each

Requires

A high-voltage electroporation apparatus capable of generating field strengths of 16 kV/cm

ElectroMAX DH10B Cells are electrocompetent E. coli cells offering the highest transformation efficiencies of >1 x 10¹⁰ cfu/μg plasmid DNA.

ElectroMAX DH10B Cells are electrocompetent E. coli cells offering the highest transformation efficiencies of >1 x 10¹⁰ cfu/μg plasmid DNA. The high transformation efficiencies make these cells ideal for cloning both prokaryotic and eukaryotic genomic DNA and efficient plasmid rescue from eukaryotic genomes.

ElectroMAX DH10B Cells are suitable for construction of gene banks, generation of cDNA libraries using plasmid-derrived vectors, and for situations when DNA is limited. They are ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction.

ElectroMAX DH10B Cells provide:
• High-efficiency transformation to maximize clones
• Enhanced genetic markers for cDNA or gDNA cloning capabilities

ElectroMAX DH10B Cell features
• Φ80lacZΔM15 marker that provides α-complementation of the β-galactosidase gene allowing blue/white screening on agar plates containing X-gal or Bluo-gal
• mcrA genotypic marker and mcrBC, mrr deletion that allow cloning DNA that contains methylcytosine and methyladenine
• endA1 mutation that enables increased plasmid yield and quantity

Genotype
F^-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139Δ(ara, leu)7697 galU galK λ^-rpsL nupG
Get more detailed information about specific Genotypes and Genetic Markers of our E. coli Competent Cells.

Find the strain and format that you need
DH10B cells are available in both electrocompetent and chemically competent formats.
ElectroMax DH10B T1R Cells (Cat. No. 12033015) and MegaX DH10B T1^R Electrocomp Cells (Cat. No. C640003) offer the benefit of T1 and T5 phage resistance.

Note: A high-voltage electroporation apparatus capable of generating field strengths of 16 kV/cm is required.

Order Info

Shipping Condition: Dry Ice

Specifications

• Product Type: Electrocompetent Cells
• Contains F' Episome: No
• Improves Plasmid Quality: Yes (endA1)
• Cloning Methylated DNA: Yes (mcrA)
• Transformation Efficiency Level: High Efficiency (>1 x 10⁹ cfu/μg)
• Content And Storage: • ElectroMAX DH10B Cells (5 x 100 μL)
  Store Competent Cells at –80°C.
  
  • pUC19 DNA (50 μL at 10 pg/μL)
  Store pUC19 DNA at –20°C.
  
  • S.O.C. medium (2 x 6 mL)
  Store S.O.C. Medium at 4°C or room temperature.
  
  
• Antibiotic Resistance Bacterial: Yes (Streptomycin)
• Cloning Unstable DNA: Not suitable for cloning unstable DNA
• Blue/White Screening: Yes (lacZΔM15)
• High-throughput Compatibility: Low
• Plasmid: May be used for plasmids >20 kb
• Preparing Unmethylated DNA: No
• Reduces Recombination: Yes (recA1)
• Shipping Condition: Dry Ice
• T1 Phage - Resistant (tonA): No
• Species: E. coli (K12)
• Format: Tube
• Product Line: ElectroMAX™
• Quantity: 5 x 100 μL

For Research Use Only. Not for use in diagnostic procedures.