Invitrogen™ Expressway™ Mini Cell-Free Expression System

Includes

Designed to perform 20 x 50μL reactions or 1 x 1mL reaction. This system includes the IVPS E.coliExtract, IVPS reaction buffer, feed buffer, 19 amino acid mix, methionine, DNase/RNase-free water, RNase A, T7 Enzyme Mix, 2mL reaction tubes, and a positive expression control vector.

Uses efficient coupled transcription and translation reaction to produce active recombinant protein

• Eliminates time-consuming steps of cell-based protein production including transformation, cell culture, and expression optimization
• In two hours, can produce protein suitable for array of downstream applications and functional studies
• Offers flexibility in experimental design
• Synthesize proteins from both circular plasmid and linear templates (e.g. PCR products)
• Perform high-throughput synthesis of proteins
• Express genes toxic to in vivosystems
• Provides all components need for optimal cell-free protein production
• As DNA template is transcribed, 5« end of mRNA is bound by ribosomes and undergoes translation as 3« end of template is still being transcribed
• Proprietary feed buffer formulation is provided with system for increased protein yields

Cell Free Protein Expression for Functional Analysis, Cell-Free (in vitro) Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Promoter

T7

Product Type

Cell-Free Expression System

Expressed Protein Type

Water Soluble Protein

Expression System

Cell-Free

Expression Mechanism

Cell-Free Expression

Quantity

1 kit

Product Line

Expressway™

For Research Use Only. Not for use in diagnostic procedures.