Invitrogen™ FreeStyle™ 293-F Cells

Complete cell culture system for generating large amounts of mammalian recombinant protein

Animal origin-free, chemically defined, low-protein, reduced growth factors, reduced serum, xeno-free. The FreeStyle 293-F cell line is derived from the 293 cell line and is intended for use with the FreeStyle MAX 293 Expression System or FreeStyle 293 Expression System. FreeStyle 293-F cells are adapted to suspension culture in FreeStyle 293 Expression Medium. Frozen cells are supplied in and may be thawed directly into FreeStyle 293 Expression Medium. The FreeStyle 293-F cell line is supplied in a vial containing 1mL of cells at 1 x 10⁷ viable cells/mL in 90% FreeStyle 293 Expression Medium and 10% DMSO

Characteristics of FreeStyle 293-F cells:

• Prepared from low-passage Master Cell Bank cultures derived from parental 293-F cells that were recloned by limiting dilution
• The 293 clonal derived cultures are maintained in serum-free conditions for only 30 to 35 total passages
• Adapted to high-density, serum-free, suspension growth and may be maintained in FreeStyle 293 Expression Medium
• Demonstrate high transfection efficiencies with FreeStyle MAX reagent
• Suspension cultures may be transfected in FreeStyle 293 Expression Medium without the need to change media
• Permits transfection of cells at large volumes
• Voltage limit: 0.5-2.5kV

Antibody and Cell Culture Production, Bioproduction, Cell Culture, Mammalian Expression, Mammalian Expression Cell Lines, Mammalian Protein Production, Plasmid Transfection, Protein Expression, Proteins, Expression, Isolation and Analysis, Transfection

Cell Line

FreeStyle™ 293-F Cells

Content And Storage

1 vial of 1 x 10⁷ cells. Store in liquid nitrogen.

Cell Type

293-F

Species

Human

For Use With (Application)

Protein Expression and Transfection

Media Adaptation

FreeStyle™ 293 Expression Medium

Product Line

Freestyle™

Product Type

293-F Cell

Quantity

1 mL

System Type

FreeStyle™

For Research Use Only. Not for use in diagnostic procedures.