Invitrogen™ β-Gal Assay Kit

Description

Includes

10X PBS (phosphate buffered saline), lysis buffer, cleavage buffer, β-mercaptoethanol, ONPG, stop buffer.

Provides reagents required to quickly measure levels of active β-galactosidase expressed in cells transfected with plasmids expressing lacZgene

• lacZis bacterial gene often used as reporter construct in eukaryotic transfection experiments
• Gene product of lacZ, β-galactosidase, is resistant to proteolysis in cellular lysates, and its activity is easily assayed
• β-galactosidase catalyzes hydrolysis of β-galactosidase such as ortho-nitrophenyl-D-galactopyranoside (ONPG)
• Hydrolysis of ONPG to ONP anion produces bright yellow color with peak absorbance at 420nm that can be quantified using spectrophotometer
• 10.7 gene expression
• Colorimetric detection

Beta-Galactosidase Assays, Enzyme and Protein Activity Assays, Proteins, Expression, Isolation and Analysis, Reporter Assays

Specifications

• Content And Storage: The β-Gal Assay Kit contains 10X PBS (phosphate buffered saline), lysis buffer, cleavage buffer, β-mercaptoethanol, ONPG, stop buffer. Store ONPG at -20°C. All other components are stored at either room temperature or +4°C. All reagents are guaranteed stable for 6 months when properly stored.
• Assay: β-Gal (lacZ) Assay
• Detection Method: Colorimetric
• Product Type: Beta-Gal Assay Kit
• Substrate: ONPG
• Quantity: 80 mL
• Target: Beta-Galactosidase

For Research Use Only. Not for use in diagnostic procedures.