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Includes
10X PBS (phosphate buffered saline), lysis buffer, cleavage buffer, β-mercaptoethanol, ONPG, stop buffer.
- lacZis bacterial gene often used as reporter construct in eukaryotic transfection experiments
- Gene product of lacZ, β-galactosidase, is resistant to proteolysis in cellular lysates, and its activity is easily assayed
- β-galactosidase catalyzes hydrolysis of β-galactosidase such as ortho-nitrophenyl-D-galactopyranoside (ONPG)
- Hydrolysis of ONPG to ONP anion produces bright yellow color with peak absorbance at 420nm that can be quantified using spectrophotometer
- 10.7 gene expression
- Colorimetric detection
Beta-Galactosidase Assays, Enzyme and Protein Activity Assays, Proteins, Expression, Isolation and Analysis, Reporter Assays
Specifications
Content And Storage
The β-Gal Assay Kit contains 10X PBS (phosphate buffered saline), lysis buffer, cleavage buffer, β-mercaptoethanol, ONPG, stop buffer. Store ONPG at -20°C. All other components are stored at either room temperature or +4°C. All reagents are guaranteed stable for 6 months when properly stored.
Assay
β-Gal (lacZ) Assay
Detection Method
Colorimetric
Product Type
Beta-Gal Assay Kit
Substrate
ONPG
Quantity
80 mL
Target
Beta-Galactosidase
For Research Use Only. Not for use in diagnostic procedures.
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