Invitrogen™ β-Gal Staining Kit

Includes

Sufficient reagents to stain fifty 60mM plates. The kit includes 10X PBS (phosphate buffered saline), X-gal, staining solutions A, B, and C, 10X fixative solution, and 10μg of pcDNA 3.1/His/lacZ control vector.

Determine percentage of transfected cells expressing lacZ

• lacZ is bacterial gene often used as reporter construct in eukaryotic transfection experiments
• Gene product of lacZ, β-galactosidase, is resistant to proteolysis in cellular lysates, and its activity is easily assayed
• β-galactosidase catalyzes hydrolysis of X-gal, producing a blue color that can be easily visualized under microscope
• Colorimetric detection

Beta-Galactosidase Assays/Enzyme and Protein Activity Assays/Proteins, Expression, Isolation and Analysis/Reporter Assays

Content And Storage

The β-Gal Staining Kit contains sufficient reagents to stain fifty 60 mm plates. The kit includes 10X PBS (phosphate buffered saline), X-gal, staining solutions A, B, and C, 10X fixative solution, and 10 μg of pcDNA™ 3.1/His/lacZ control vector. Store the 10X PBS at room temperature. All other components should be stored at -20°C. All reagents are guaranteed stable for 6 months when properly stored.

Assay

β-Gal (lacZ) Assay

Detection Method

Colorimetric

Product Type

Beta-Gal Staining Kit

Substrate

X-Gal

Quantity

1 kit

Target

Beta-Galactosidase

For Research Use Only. Not for use in diagnostic procedures.