Invitrogen™ Baculovirus Expression System with Gateway™ Technology

Designed to create recombinant pFastBac™ plasmids containing polyhedrin promoter

Supplier:  Invitrogen™ 11827011

Catalog No. 11-827-011

Includes: LR Clonase enzyme mix and reaction buffer, destination vectors pDEST8, pDEST10, and pDEST20, proteinase K solution (2μg/μL), pENTR™-gus positive control, and Library Efficiency DH5Competent Cells. The kit does not include MAX Efficiency DH10Bac Competent Cells.

Description

• Destination vectors carrying polyhedrin promoter for production of native (pDEST™8), N-terminal histidine fusion (pDEST™10), and N-terminal GST fusion (pDEST™20) proteins
• pDEST™10 vector containing TEV protease cleavage site for removal of fusion tag after protein purification

Baculovirus Production and Titering/Insect Expression/Protein Expression/Proteins, Expression, Isolation and Analysis

Specifications

• Promoter: Polyhedrin
• Content And Storage: The Baculovirus Expression System with Gateway™ Technology includes LR Clonase™ enzyme mix and reaction buffer, destination vectors pDEST™8, pDEST™10, and pDEST™20, proteinase K solution (2 μg/μl), pENTR™-gus positive control, and Library Efficiency™ DH5Competent Cells. The kit does not include MAX Efficiency™ DH10Bac™ Competent Cells. Store LR Clonase™ enzyme mix and Competent Cells at -80°C. Store all other components at -20°C. Components are guaranteed stable for 6 months when properly stored.
• Cloning Method: Gateway
• Vector: pDEST, Gateway Baculovirus Vectors

• Product Type: Expression System
• Protein Tag: GST Tag, His Tag (6x), Untagged
• Quantity: 20 reactions
• Product Line: Gateway™

For Research Use Only. Not for use in diagnostic procedures.