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Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ TOP10 Chemically Competent E. coli (Supply Center Only)
Includes
TOPO-adapted, ready-to-use pCR8/GW/TOPO Vector, dNTPs, Salt Solution, Sterile Water, GW Forward and Reverse Sequencing Primers, Control Template and PCR Primers, TOP10 Chemically Competent E. coli or Mach1-T1R Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid. The PureLink Quick Plasmid Miniprep Kit contains Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffer, TE Buffer, Spin Columns, and Wash and Recovery Tubes.
- Use pCR™-TOPO Vector with covalently bound topoisomerase I for fast cloning and recombinants
- Comes with Gateway (GW) entry vector, pCR™8/GW/TOPO
- Vector has 3'-T overhangs for direct ligation of Taq-amplified PCR products
- att sites for rapid recombination into variety of Gateway destination vectors
- Novel GW primer sites, within the att regions, located less than 55 base pairs from the PCR product insertion site for convenient sequencing
- Spectinomycin resistance gene for robust selection in E. coli
- EcoR I sites flanking PCR product insertion site for easy excision of inserts
Cloning,Gateway Cloning,PCR Cloning
Order Info
Shipping Condition: Dry Ice
Specifications
Cloning Method
TOPO™ with Gateway™
Content And Storage
The pCR™8/GW/TOPO™ TA Cloning Kit contains the TOPO™-adapted, ready-to-use pCR™8/GW/TOPO™ Vector, dNTPs, Salt Solution, Sterile Water, GW Forward and Reverse Sequencing Primers, Control Template and PCR Primers, One Shot™ TOP10 Chemically Competent E. coli or One Shot™ Mach1™-T1R Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid. The PureLink™ Quick Plasmid Miniprep Kit contains Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffer, TE Buffer, Spin Columns, and Wash and Recovery Tubes. Store all components of the PureLink™ kit at room temperature. Store the One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
No. of Reactions
20 reactions
Product Line
One Shot™
Product Type
TA Cloning Kit
Shipping Condition
Dry Ice
Vector
pCR
For Research Use Only. Not for use in diagnostic procedures.
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