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Includes
6μg pDONR221 vector, lyophilized in TE buffer, pH 8.0.
- Designed to create entry clone following PCR with attB-modified custom primers
- With entry clone, recombine gene of interest into variety of expression vectors adapted for use in Gateway technology
- PCR product is directionally cloned with efficiencies typically >99%
- Rapid, efficient, directional cloning of PCR products
- Versatility to use any amplification enzyme
- Cloning without need for restriction endonucleases or ligase
- Selection of entry clones with kanamycin or Zeocin™
- In addition, pDONR221 and pDONR™/Zeo vectors have pUC origin for high plasmid yields and universal M13 sequencing sites for ease of use
Cloning, Gateway Cloning
Specifications
Product Type
Gateway System Destination Expression Vector
Content And Storage
6 μg pDONR221™ vector, lyophilized in TE buffer, pH 8.0. Upon receipt, store at -20°C.
Cloning Method
Gateway
Quantity
6 μg
Vector
pDONR
Product Line
Gateway™, pDONR™
For Research Use Only. Not for use in diagnostic procedures.
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