Invitrogen™ pTrcHis2 A, B, & C Bacterial Expression Vectors

Includes

All vectors are supercoiled and are provided lyophilized. A TOP10 E. coli stab and positive expression control are provided.

Offer enhanced translation initiation and high-level expression in E. coli

• These vectors feature high-level regulated transcription from the trc promoter
• Enhanced translation efficiency of eukaryotic genes in E. coli
• The lacO operator and lacIq repressor gene for transcriptional regulation in any E. coli strain
• This particular vector offers C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an anti-HisG antibody
• C-terminal Xpress epitope for easy detection with an anti-Xpress antibody
• Enterokinase cleavage site for removal of fusion tag

Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Constitutive or Inducible System

Inducible

Inducing Agent

IPTG

Promoter

Trc, lacO

Product Type

Bacterial Expression Vector

Selection Agent (Eukaryotic)

None

Content And Storage

All vectors are supercoiled and are provided lyophilized. A TOP10 E. coli stab and positive expression control are provided. Store vectors at -20°C. Store stab at room temperature. The vector is guaranteed stable for 6 months when properly stored.

Antibiotic Resistance Bacterial

Ampicillin (AmpR)

Cleavage

EK (Enterokinase) Recognition Site

Protein Tag

Thioredoxin

Cloning Method

Restriction Enzyme/MCS

Quantity

20 μg

Vector

pTrc

For Research Use Only. Not for use in diagnostic procedures.