Invitrogen™ One Shot™ ccdB Survival™ 2 T1^R Competent Cells

Includes

ccdB Survival2 T1R Competent Cells: 11 vials, 50μL each, pUC19 DNA (10pg/μL): 1 vial, 50μL, S.O.C. medium: 1 bottle, 6mL

One Shot ccdB Survival 2 T1^R Competent Cells are suitable for the propagation of plasmids containing the ccdB gene and are designed for use with the Gateway Vector Conversion System and for propagating Gateway destination, donor, and supercoiled entry vectors.

One Shot ccdB Survival 2 T1^R Competent Cells are suitable for the propagation of plasmids containing the ccdB gene and are designed for use with the Gateway Vector Conversion System and for propagating Gateway destination, donor, and supercoiled entry vectors. ccdB Survival 2 T1R cells that are made chemically competent can achieve high transformation efficiency of >1 x 10⁹ transformants/μg plasmid DNA.

The ccdB Survival 2 T1^R E. coli strain is derived from the TOP10 strain and offers the same benefits. Mutations in the methylation-dependent restriction system (mcrA, mcrBC, and mrr) allow cloning of both prokaryotic and eukaryotic genomic DNA that contains methylcytosine and methyladenine. This strain also has the lacZΔM15 genotype, providing the option of blue-white screening on plates containing either X-Gal or Bluo-Gal. In addition, recA1 and endA1 mutations increase insert stability and improve the quality of plasmid DNA prepared from minipreps.The tonA (also known as fhuA) mutation in ccdB Survival 2 T1^R cells confers resistance to bacteriophages T1 and T5, safeguarding samples against contamination.

ccdB Survival 2 T1^R Competent Cells offer:
• >1 x 10⁹ transformants/μg efficiency
• tonA (fhuA) genotype to confer resistance to T1 and T5 phage
• Support of high-yield preparation of supercoiled plasmid DNA (the endA1 phenotype)
• Reduced nonspecific recombination of cloned DNA (the recA1 phenotype) 

Convenient and efficient format
One Shot ccdB Survival 2 T1^R E. coli are supplied in the convenient, single-reaction One Shot format. Each tube contains enough cells for one transformation so there are no efficiency-zapping, freeze-thaw cycles or money wasted on unused cells.

Genotype: F^-mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araΔ139 Δ(ara-leu)7697 galU galK rpsL(Str^R) endA1 nupG fhuA::IS2

Find the strain and format that fits your needs
• We offer other DH strains in chemically competent and electrocompetent cell formats to meet your specific needs.
• MultiShot formats for high throughput applications are available.

Order Info

Shipping Condition: Dry Ice

Product Type

Chemically Competent Cells

Contains F' Episome

No

Improves Plasmid Quality

Yes (endA1)

Cloning Methylated DNA

Yes (mcrA)

Transformation Efficiency Level

High Efficiency (>1 x 10⁹ cfu/μg)

Content And Storage

• One Shot ccdB Survival 2 T1R Competent Cells (11 x 50 μL)
Store Competent Cells at &ndash80°C.

• pUC19 DNA (50 μL at 10 pg/μL)
Store pUC19 DNA at –20°C.

• S.O.C. Medium (6 ml)
Store S.O.C. Medium at 4°C or room temperature.

Antibiotic Resistance Bacterial

Yes (Streptomycin)

Cloning Unstable DNA

Not suitable for cloning unstable DNA

Blue/White Screening

Yes (lacZΔM15)

High-throughput Compatibility

Low

Plasmid

ccdB vector propagation

Preparing Unmethylated DNA

No

Reduces Recombination

Yes (recA1)

Shipping Condition

Dry Ice

T1 Phage - Resistant (tonA)

Yes

Species

E. coli (K12)

Format

Tube

Product Line

One Shot™

Quantity

11 x 50 μL

For Research Use Only. Not for use in diagnostic procedures.