Invitrogen™ One Shot™ TOP10F' Chemically Competent E. coli

One Shot TOP10F' Chemically Competent E. coli are identical to TOP10 cells, with the addition of an F' episome.

One Shot TOP10F´ Chemically Competent E. coli are identical to TOP10 cells, with the addition of an F´ episome. The F´ episome carries a tetracycline resistance gene and allows isolation of single-stranded DNA (ssDNA) from vectors that have an f1 origin of replication. In addition, the F´ episome carries the lacIq repressor for inducible expression from trc, tac, and lac promoters using IPTG.

TOP10F´ competent cells are provided at a transformation efficiency of >1 x 10⁹ cfu/μg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation.

As a derivative of TOP10, TOP10F´ Chemically Competent E. coli cells carry mutations in the methylation-dependent restriction system (mcrA, mcrBC, and mrr) allowing them to clone both prokaryotic and eukaryotic genomic DNA and provide efficient plasmid rescue from eukaryotic genomes. TOP10F´ cells also carry recA1 and endA1 mutations that increase insert stability and improve the quality of plasmid DNA prepared from minipreps.

One Shot TOP10F´ Chemically Competent E. coli cells offer:
• Transformation efficiencies of >1 x 10⁹ cfu/μg
• Stable F´ episome that allows isolation of ssDNA and carries lacIq repressor for inducible expression from trc, tac, and lac promoters
• hsdR for efficient transformation of unmethylated DNA from PCR amplifications
• mcrA for efficient transformation of methylated DNA from genomic preparations
• lacZΔM15 for blue/white color screening of recombinant clones on plates containing X-Gal or Bluo-Gal
• endA1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I
• recA1 for reduced occurrence of nonspecific recombination in cloned DNA

Note: IPTG induction is required for blue-white color screening.

Easy-to-use One Shot format
TOP10 F´ Chemically Competent E. coli cells are supplied in the convenient, single-reaction One Shot format. The single-tube, single-use format allows all steps of the transformation protocol, up to plating, to take place in the same tube, saving time and preventing contamination.

Genotype
F´{lacIq, Tn10(TetR)} mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL(StrR) endA1 nupG

Find the strains and formats that fit your needs
• DH strains are available in chemically competent and electrocompetent cell formats.
• For high throughput applications there are a variety of TOP10 strains in MultiShot formats.
• For ssDNA production try strains such as TOP10F´ (Cat. No. C303003) or electrocompetent DH12S (Cat. No. 18312017).

Order Info

Shipping Condition: Dry Ice

Product Type

Chemically Competent Cells

Contains F' Episome

Yes

Improves Plasmid Quality

Yes (endA1)

Cloning Methylated DNA

Yes (mcrA)

Transformation Efficiency Level

High Efficiency (>1 x 10⁹ cfu/μg)

Content And Storage

• One Shot TOP10F' Chemically Competent E. coli (21 x 50 μL)
Store Competent Cells at –80°C.

• pUC19 plasmid (50 μL at 10 pg/μL)
Store pUC19 plasmid at –20°C.

• S.O.C. Medium (6 mL)
Store S.O.C. Medium at 4°C or room temperature.

Antibiotic Resistance Bacterial

Yes (Streptamycin, Tetracycline)

Cloning Unstable DNA

Not suitable for cloning unstable DNA

Blue/White Screening

Yes (lacZΔM15)

High-throughput Compatibility

Low

Preparing Unmethylated DNA

No

Reduces Recombination

Yes (recA1)

Shipping Condition

Dry Ice

T1 Phage - Resistant (tonA)

No

Species

E. coli (K12)

Promoter

Trc, Tac, Lac

Format

Tube

Product Line

One Shot™

Quantity

21 x 50 μL

For Research Use Only. Not for use in diagnostic procedures.