Invitrogen™ pBAD TOPO™ TA Expression Kit

Includes

Each pBAD TOPO TA Expression Kit contains two boxes and the LMG194 E. coli stab. The pBAD TOPO TA box contains all of the reagents necessary for PCR (except Taq polymerase), including 200ng of topoisomerase I-activated pBAD-TOPO vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, 20% L-arabinose, pBAD primers for sequencing or PCR screening, and an expression control plasmid. The other box contains all of the reagents necessary for transformation, including single-use 50μL aliquots of TOP10 Chemically Competent E. coli, S.O.C. medium, and a supercoiled control plasmid.

Specifically designed for one-step cloning and regulated prokaryotic expression of Taq-amplified PCR products

• Linearized, topoisomerase I-activated vector for 5-minute cloning of Taqamplified PCR products
• araBAD promoter for tightly regulated expression in E. coli V5 epitope tag for detection with Anti-V5 Antibody
• C-terminal polyhistidine (6xHis) tag for purification using nickel-chelating resin and detection with Anti-His(C-term) Antibody
• Enterokinase cleavage site for removal of N-terminal leader peptide

Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Constitutive or Inducible System

Inducible

Inducing Agent

Arabinose

Promoter

araBAD

Product Type

TA Expression Kit

Selection Agent (Eukaryotic)

None

Content And Storage

Each pBAD TOPO™ TA Expression Kit contains two boxes and the LMG194 E. coli stab. The pBAD TOPO™ TA box contains all of the reagents necessary for PCR (except Taq polymerase), including 200 ng of topoisomerase I-activated pBAD-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, 20% L-arabinose, pBAD primers for sequencing or PCR screening, and an expression control plasmid. Store at -20°C. The One Shot™ box contains all of the reagents necessary for transformation, including single-use 50 μl aliquots of One Shot™ TOP10 Chemically Competent E. coli, S.O.C. medium, and a supercoiled control plasmid. Store One Shot™ cells at -80°C. Store the LMG194 E. coli stab at at 2 - 8°C. All reagents are guaranteed stable for 6 months when properly stored.

Antibiotic Resistance Bacterial

Ampicillin (AmpR)

Bacterial or Yeast Strain

LMG194, TOP10

Cleavage

EK (Enterokinase) Recognition Site

Expression System

E. coli

Expression Mechanism

Cell-Based Expression

Protein Tag

V5 Epitope Tag

Cloning Method

TOPO-TA

Quantity

40 Reactions

Vector

pBAD

Product Line

TOPO™

For Research Use Only. Not for use in diagnostic procedures.