Invitrogen™ pcDNA™3.1 ⁽⁺⁾ Mammalian Expression Vector

Includes

20μg of this pcDNA3.1⁽⁺⁾ vector, as well as an expression control, are supplied supercoiled and lyophilized.

Designed for high-level, constitutive expression in a variety of mammalian cell lines

• Contains Geneticin selectable marker and reverse-orientation multiple cloning site
• For use alone or in co-transfection
• Cytomegalovirus (CMV) enhancer-promoter for high-level expression
• Large multiple cloning site in forward (+) orientation
• Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence for enhanced mRNA stability
• SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (i.e.,COS-1 and COS-7)
• Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli

Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Delivery Type

Transfection

Promoter

CMV

Product Type

Mammalian Expression Vector

Selection Agent (Eukaryotic)

Geneticin™ (G-418)

Content And Storage

20 μg of this pcDNA™3.1⁽⁺⁾ vector, as well as an expression control, are supplied supercoiled and lyophilized. Store at -20°C. Vectors are guaranteed stable for 6 months when properly stored.

Protein Tag

Untagged

Cloning Method

Restriction Enzyme/MCS

Quantity

20 μg

Vector

pcDNA

Product Line

pcDNA™

For Use With (Application)

Constitutive Expression

For Research Use Only. Not for use in diagnostic procedures.