Invitrogen™ PureLink™ Quick Gel Extraction and PCR Purification Combo Kit

Includes

The PureLink Quick Gel Extraction & PCR Purification Combo Kit includes sufficient PureLink columns, collection tubes, elution tubes, and reagents for 50 preps.

Designed to purify DNA fragments from agarose gels

Molecular biology grade

• Offers ability to perform both a gel extraction or PCR purification with single kit
• Simple procedure uses silica-based spin cartridge to purify DNA fragments from 40bp to 10kb in < 30 minutes
• Provides all components to perform rapid and efficient removal of short primers, dNTPs, enzymes, short-failed PCR products, and salts from PCR fragments >100 bp in less than 10 minutes
• Isolated DNA using PureLink kit is free of proteins, dye, and agarose, and is ready-to-use for variety of applications, including automated fluorescent DNA sequencing, manual DNA sequencing, PCR, in vitro transcription, restriction mapping, cloning, and labeling
• Contains all buffers fromPureLink Quick Gel extraction kit and PCR binding buffer from PureLink PCR Purification kit with Invitrogen lidded column
• Purification time is 20 minutes

Agarose Gel Extraction, DNA & RNA Purification & Analysis, DNA Extraction

Order Info

Shipping Condition: Room Temperature

Content And Storage

• 2 x 90 mL Gel Solubilization Buffer L3; room temperature
• 15 mL Binding Buffer B2; room temperature
• 16 mL Wash Buffer W1; room temperature
• 15 mL Elution Buffer E1; room temperature
• 50 PureLink Clean-up Spin Columns with Collection Tubes; room temperature
• 50 PureLink Elution Tubes; room temperature

Isolation Technology

Silica Spin Column

Sample Type

Linear DNA fragments from: Agarose Gels, PCR Purification, Restriction Enzyme Reactions, PCR Products

Scale

Mini

Elution Volume

50 μL

Final Product Type

DNA

For Use With (Application)

PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization

High-throughput Compatibility

Not High-throughput Compatible (Manual)

Label or Dye

Ethidium Bromide, SYBR Safe

Quantity

50 Preps

Shipping Condition

Room Temperature

Test Time

Up to 30 min.

Yield

40 μg (Binding capacity)

Starting Material Amount

Agarose Gel: ≤400 mg
PCR Product: ≤100 μL, or ≤40 μg dsDNA

For Research Use Only. Not for use in diagnostic procedures.