Invitrogen™ pYES2.1 TOPO™ TA Yeast Expression Kit

Includes

Each pYES2.1 TOPO TA Expression Kit contains two boxes. The pYES2.1 TOPO TA box contains all of the reagents necessary for ligation, including 200ng of topoisomerase I-activated pYES2.1/V5-His-TOPO vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, GAL1 Forward and V5 C-term Reverse Primers for sequencing or PCR screening, and an expression control plasmid. The other box contains all of the reagents necessary for transformation, including 21 x 50μL aliquots of chemically competent TOP10F' E. coli, S.O.C. medium, and supercoiled plasmid control pUC19.

Offers direct cloning of Taq-amplified PCR product into a Saccharomyces cerevisiae expression vector

• Uses linearized, topoisomerase I-activated pYES2.1/V5-His-TOPO vector for 5-minute cloning on bench top and produces >85% recombinants
• pYES2.1/V5- His-TOPO vector features 2μ origin of replication for high-copy maintenance gene for auxotrophic selection on economical minimal media
• C-terminal V5 epitope and polyhistidine (6xHis) tags for efficient detection and purification

Protein Expression, Proteins, Expression, Isolation and Analysis, S. cerevisiae Expression Systems, Yeast Expression

Promoter

GAL1

Product Type

TA Yeast Expression Kit

Content And Storage

Each pYES2.1 TOPO™ TA Expression Kit contains two boxes. The pYES2.1 TOPO™ TA box contains all of the reagents necessary for ligation, including 200 ng of topoisomerase I-activated pYES2.1/V5-His-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, GAL1 Forward and V5 C-term Reverse Primers for sequencing or PCR screening, and an expression control plasmid. Store at -20°C. The One Shot™ Box contains all of the reagents necessary for transformation, including twenty-one 50 μl aliquots of chemically competent TOP10F´ E. coli, S.O.C. medium, and supercoiled plasmid control pUC19. Store at -80°C. All components are guaranteed stable at the indicated temperature for 6 months when properly stored.

Antibiotic Resistance Bacterial

Ampicillin (AmpR)

Protein Tag

His Tag (6x), V5 Epitope Tag

Cloning Method

TOPO-TA

Quantity

20 reactions

Vector

pYES, TOPO-TA Cloning Vectors

Product Line

TOPO™, YES™

For Research Use Only. Not for use in diagnostic procedures.