Invitrogen™ RNaseOUT™ Recombinant Ribonuclease Inhibitor

Protects mRNA and improves total cDNA yields

RNaseOUT Recombinant Ribonuclease Inhibitor is a potent noncompetitive inhibitor of pancreatic-type ribonucleases such as RNase A, and is used to avoid RNA degradation in a variety of applications. RNaseOUT Recombinant Ribonuclease Inhibitor is an acidic protein with a molecular weight of approx. 52 kDa. RNaseOUT inhibits RNase A, RNase B, and RNase C.
Applications cDNA synthesis, RT-PCR, and in vitro transcription and translation
Source Purified by affinity chromatography from E. coli expressing a cloned porcine gene
Performance and quality testing SDS-PAGE purity, endodeoxyribonuclease assay, protein concentration, specific activity, performance evaluated by RT-PCR
Unit definition One unit inhibits 5 ng of RNase A by 50% using cytidine 2', 3' cyclic monophosphate (cCMP) as the substrate
Unit reaction conditions 100mM Tris-acetate (pH 6.5), 1mM EDTA, 0.2mM cCMP, 2 μg RNase A in 1mL from 0 to 10 min at 25°C

PCR and Real-Time PCR, Reverse Transcription

Order Info

Shipping Condition: Dry ice

Concentration

100 mM

Content And Storage

Store at -20°C. Avoid exposing product to frequent changes in temperature. RNaseOUT™ Ribonuclease Inhibitor requires 1 mM DTT to maintain activity.

The product is guaranteed for 6 months from date of purchase unless otherwise stated in the product documentation.

Purification Time

10 min.

Product Line

RNaseOUT™

Quantity

5000 units

Shipping Condition

Dry Ice

For Research Use Only. Not for use in diagnostic procedures.