Invitrogen™ T4 DNA Ligase (1 U/μL)

Ideal for cloning (blunt-end or cohesive-end ligation) and adding linkers or adapters to blunt-ended DNA

Supplier:  Invitrogen™ 15224017

Catalog No. 15-224-017

Includes: Vial of 5X reaction buffer (250mM Tris-HCl (pH 7.6), 50mM MgCl₂, 5mM ATP, 5mM DTT, 25% (w/v) polyethylene glycol-8000)

Description

T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3'hydroxyl and 5'phosphate termini. Single-stranded nucleic acids are not substrates for this enzyme.

• Optimizes ligation in 5 minutes
• Source: Purified from E. coli lambda lysogen NM989
• Performance and quality testing: Endodeoxyribonuclease, 3' and 5' exodeoxyribonuclease assays; ligation efficiency tested
• Unit definition: One unit catalyzes exchange of 1nmol ³²P-labeled pyrophosphate into ATP in 20 min. at 37°C. One unit is equal to approximately 300 cohesive-end ligation units
• Unit reaction conditions: 66mM Tris-HCl (pH 7.6), 6.6mM MgCl₂, 10mM DTT, 66μM ATP, 3.3μM ³²P-labeled pyrophosphate, and enzyme in 0.1mL for 20 min. at 37°C

ChIP-on-Chip, Chromatin Biology, Cloning, RNAi, Epigenetics & Noncoding RNA Research, Restriction Enzyme Cloning

Order Info

Shipping Conditions: Dry ice

Specifications

• Concentration: 1 U/μL
• Shipping Condition: Dry Ice
• Compatible Buffer: DNA Ligase Buffer, 5X Reaction Buffer
• Product Type: T4 DNA Ligase

• Content And Storage: T4 DNA Ligase is supplied with a vial of 5X reaction buffer [250 mM Tris-HCl (pH 7.6), 50 mM MgCl₂, 5 mM ATP, 5 mM DTT, 25% (w/v) polyethylene glycol-8000]. Store at -20°C.
• Enzyme: Ligase
• Quantity: 100 U

For Research Use Only. Not for use in diagnostic procedures.