Invitrogen™ TOPO™ TA, Dual TOP10 (Supply Center Packaging)

For fast, efficient cloning and subsequent in vitro transcription/translation

Supplier:  Invitrogen™ K460001SC

Catalog No. K460001SC

Includes: Topoisomerase I-activated pCR™II-TOPO™ vector; PCR buffer; Salt solution; dNTPs; Control template; T3, T7, M13 forward, and M13 reverse primers; Control PCR primers; Sterile water; One Shot™ Chemically Competent or Electrocomp™ E. coli; S.O.C. medium; Supercoiled pUC19 control plasmid

Description

This kit includes the pCR™II-TOPO TA vector. By eliminating time-consuming and tedious restriction site cloning, TOPO cloning is the most reliable method, taking only 5 minutes using a 3-step protocol, and yielding up to 95% recombinants. This product comes in Supply Center packaging, which features an additional outer box that holds both the TOPO vector box and the competent cells box. This special packaging helps ensure that visitors to a Supply Center obtain the complete kit.

• 3'-T overhangs for direct ligation of Taq-amplified PCR products
• T7 and SP6 promoters for efficient in vitro transcription
• M13 forward and reverse primer sites for sequencing
• 16 convenient restriction sites, including EcoRI, flanking your insert for subsequent excision or subcloning
• Kanamycin and ampicillin resistance for your choice of selection in E. coli
• Easy blue/white colony screening for selection of recombinants
• Includes OneShot™ TOP10 Competent cells for added convenience and highest cloning efficiencies

Cloning, PCR Cloning

Order Info

Shipping Condition: Dry Ice

Specifications

• Bacterial or Yeast Strain: TOP10
• Content And Storage: Box 1:
  • Topoisomerase I-activated pCR™II-TOPO™ vector
  • PCR buffer
  • Salt solution
  • dNTPs
  • Control template
  • M13 forward and reverse primers
  • Control PCR primers
  • Sterile water
  
  Store at -5 to -30°C.
  All reagents are stable for 6 months when properly stored.
  
  Box 2:
  • One Shot™ Chemically Competent or Electrocomp™ E. coli
  • S.O.C. medium
  • Supercoiled pUC19 control plasmid.
  
  Store at -68 to -85°C.
• Product Type: Cloning Promoter Kit
• Shipping Condition: Dry Ice

• Cloning Method: TOPO™-TA
• Product Line: One Shot™
• Quantity: 25 Reactions
• Vector: TOPO-TA Cloning Vectors

For Research Use Only. Not for use in diagnostic procedures.