Thermo Scientific™ L-Proline for SILAC

Study protein expression by mass spectrometry with mass variants of lysine, arginine and other amino acids to perform stable isotope labeling with amino acids in cell culture.

Thermo Scientific™ Heavy and Light Amino Acids are used to specifically analyze protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC) quantification kits.

SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of “heavy“ ¹³C- or ¹⁵N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.

Highlights:

• Efficient – 100% label incorporation into proteins of living cells

• Flexible – different isotopes of heavy and/or light amino acids for arginine, lysine, leucine and proline enable the quantitation of peptides derived from MS-grade proteases

• Multiplex capabilities – several alternative isotopes of arginine and lysine are available that allow the analysis of multiple treatment conditions in each experiment

• High-quality supplements – heavy amino acids with >99% isotope purity

Description

L-Proline

For Use With (Equipment)

Mass Spectrometer

Product Type

Amino Acids

Content And Storage

Store at room temperature.

Label or Dye

Mass Isotope of Amino Acid

Shipping Condition

Room Temperature

Starting Material

Cells

Final Product Type

Protein

Detection Method

Mass Spectrometry

Workflow Step

Protein Labeling

Quantity

115 mg

Form

Powder

For Research Use Only. Not for use in diagnostic procedures.