Legionella pneumophila subsp. pneumophila (strain Philadelphia 1/ATCC 33152/DSM 7513) sdeA Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA5117540

Catalog No. PIPA5117540

Description

This antibody has been tested in direct-ELISA

Secreted effector that interferes with the host cell ubiquitin pathway and is required for intracellular bacterial replication. Catalyzes the ubiquitination of several mammalian Rab proteins (Rab33b, Rab1, Rab6a and Rab30) during L.pneumophila infection, without engaging the standard cellular enzyme cascade (E1 and E2). Transfers an ADP-ribose moiety from NAD to the 'Arg-42' residue of ubiquitin in a reaction that releases nicotinamide. The modified ubiquitin is subsequently transferred to the substrate protein through an unknown mechanism that results in the release of AMP. Cannot ubiquitinate the endosomal Rab5 or the cytoskeletal small GTPase Rac1 (PubMed:27049943). Also acts as a deubiquitinase (DUB), catalyzing the cleavage of three of the most abundant polyubiquitin chains ('Lys-11', 'Lys-48' and 'Lys-63') with a distinct preference for 'Lys-63' linkages; is thus able to efficiently remove 'Lys-63'-linked polyubiquitin chains from the phagosomal surface. Is also able to remove NEDD8 from neddylated proteins, but is unable to recognize SUMO. The DUB activity of SdeA is important for regulating the dynamics of ubiquitin association with the bacterial phagosome, but is dispensable for its role in intracellular bacterial replication (PubMed:26598703).

Specifications

• Antigen: Legionella pneumophila subsp. pneumophila (strain Philadelphia 1/ATCC 33152/DSM 7513) sdeA
• Classification: Polyclonal
• Conjugate: Unconjugated
• Host Species: Rabbit
• Purification Method: Protein G
• Regulatory Status: RUO
• Target Species: Bacteria
• Product Type: Antibody
• Isotype: IgG

• Applications: Western Blot
• Concentration: 2.88 mg/mL
• Formulation: 10mM PBS with 50% glycerol and 0.03% ProClin 300; pH 7.4
• Immunogen: Recombinant Legionella pneumophila subsp Ubiquitinating/deubiquitinating enzyme SdeA protein (19-268aa)
• Quantity: 100 μL
• Primary or Secondary: Primary
• Content And Storage: -20°C or -80°C if preferred
• Form: Liquid