Invitrogen™ Lens Culinaris (Edible Lentil) Agglutinin (LCA), fluorescein (FITC)

Lens Culinaris Agglutinin (LCA), fluorescein ( FITC), is a bright-green LCA-fluorophore conjugate that is sourced from a common edible lentil.

LCA specificity is similar to concanavalin A, but narrower, resulting in differences in patterns of tissue staining. Wheat germ agglutinin, concanavalin A, and LCA are known to block inhibitory effects of nerve growth factor in cell-free systems.

• Very bright FITC conjugate detected with traditional FITC/GFP filter set, Ex/Em: 495/515 nm
• Applications: immunofluorescence, glycobiology
• Sugar specificity: mannose, glucose
• Inhibiting/eluting sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside
• Blood group specificity: non-specific
• Unconjugated lectin molecular weight: 50 KDa
• Immunofluorescence working concentration: 5–20 μg/mL

LCA-FITC is provided at a 5 mg/mL concentration in 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM Ca²⁺.

If a precipitate forms upon long-term storage at 4°C, warm to 37°C and centrifuge before use.

Conjugate

FITC

Quantity

5 mg

Source

Lentil

Content And Storage

When stored at or below 2°C to 8°C in the dark, the product is stable for 6 months after receipt.

Shipping Condition

Approved for shipment at Room Temperature or on Wet Ice

Form

Solution

Protein Family

Lectins

For Research Use Only. Not for use in diagnostic procedures.