Cytiva Lentil Lectin Sepharose™ 4B Medium

Routinely used in the preparation and purification of a number of glycoproteins and carbohydrate-containing molecules

• Lentil lectin coupled to Sepharose 4B via cyanogen bromide activation
• Lentil lectin is a metalloprotein, containing Ca2+ and Mn2+, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars
• Retains binding in 1% deoxycholate; especially suited for purification of detergent-solubilized membrane proteins
• Ligand concentration 2 mg Lentil lectin/ml; binding capacity for porcine thyroglobulin approx. 16mg/mL medium
• Average particle size 90μm
• Stable in the pH range 3-10, and in the presence of all commonly used buffers (Chelating agents and 8M urea to be avoided)
• Has more equal affinity for α-D-mannose and α-D-glucose compared to Con A

Order Info

Supplied in 20% ethanol containing 0.9% NaCl, 1mM Ca²⁺ and 1mM Mn²⁺

Binding Capacity

25mg/mL

Ligand Concentration

2mg Lentil lectin/mL Medium

Matrix

4% Agarose

Particle Size

90 μm

pH

3 to 10

Quantity

25 mL

Content And Storage

2°C to 8°C

For Use With (Application)

Used for purification of a number of Glycoproteins and other Carbohydrate containing molecules

Ligand

Lentil lectin