Invitrogen™ LIVE/DEAD™ Fixable Near-IR Dead Cell Stain Kit, for 633 or 635 nm excitation

Determines the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation.

• Used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation
• Kit has been optimized and validated for use with a red laser flow cytometer
• Conveniently packaged in 40-test vials to help ensure the stability and performance of the dye over time
• Amine reactive dyes in solution will lose their effectiveness over a short period of time, therefore it is recommended to completely use the vial once rehydrated
• If this is not possible, aliquot the vials in small volumes and store at -80°C, avoiding freeze-thaw cycles
• LIVE/DEAD™ Fixable Dead Cell Stains are available in a wide variety of colors to meet multi-color panel needs

Optimal brightness

• The LIVE/DEAD™ Fixable Near-IR stain was selected based on its fluorescent properties to provide a bright signal when excited with a red laser
• The near-IR fluorescent reactive dye has an excitation maximum of ∼633 nm making it ideal for use with the red or HeNe laser with an emission of ∼750 nm
• Since live and dead cells can be discriminated using a single dye and a single channel of a flow cytometer, it is an ideal choice for multi-color experiments

Product Line

LIVE/DEAD™

Quantity

80 assays

Content And Storage

Contains 2 vials of LIVE/DEAD™ fixable dead cell stain and 500 μL DMSO. Store at -20°C.

Detection Method

Fluorescent

Dye Type

LIVE/DEAD™ FixableNear-IR Dead Cell Stain

Shipping Condition

Room Temperature

Emission

750/775

Format

Tube(s)

Form

Solid

For Research Use Only. Not for use in diagnostic procedures.