Invitrogen™ MagicMark™ XP Western Protein Standard

Includes

250 μL of ready-to-use standard mixture is provided in a plastic vial; Loading buffer consists of 125 mM Tris-HCl (pH 6.8), 10 mM DTT, 17.4% Glycerol, 3% SDS, and 0.025% Bromophenol Blue

Specifically designed for easy and convenient protein molecular weight estimation directly on Western blots

• MagicMark XP Western Protein Standard consists of nine recombinant proteins (20–220 kDa), each of which contains an IgG binding site
• The IgG binding site binds the primary or secondary antibody used for detection of the target protein, allowing direct visualization of the standard on the western blot
• The MagicMark XP Standard is compatible with most western kits and substrates (chemiluminescent, chromogenic, and fluorescent)
• The protein standard is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use
• Store at -20°C

Applications

• Western blotting: detection of the nine unstained bands via the detection method used for the target protein. Compatible with chemiluminescent substrates and fluorescent secondary antibodies (not recommended for antibodies labeled with fluors in the 500–550 nm channel)
• Protein size estimation: protein sizing on SDS-polyacrylamide gels and on western blot using western blot detection method

Order Info

Shipping Condition: Dry Ice

Content And Storage

250 μL of ready-to-use standard mixture is provided in a plastic vial. Loading buffer consists of 125 mM Tris-HCl (pH 6.8), 10 mM DTT, 17.4% Glycerol, 3% SDS, and 0.025% Bromophenol Blue.

Store at -20°C.

Detection Method

User defined detection system

Number of Markers

9

Product Type

Protein Ladder

Ready to Load

Yes

Size Range

20 to 220 kDa

Stain Type

Unstained

Gel Compatibility

Bolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels

Molecular Weight (g/mol)

220, 120, 100, 80, 60, 50, 40, 30, 20 kDa

Product Line

MagicMark™

Quantity

250 μL

Shipping Condition

Dry Ice

System Type

Western Blotting, SDS-PAGE

For Research Use Only. Not for use in diagnostic procedures.