Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix

Provide one-tube master mix for convenient and consistent cDNA synthesis for two-step RT-qPCR applications. The format helps streamline the RT-qPCR workflow, saves time, reduces pipeting errors to give customers sensitive, reliable and fast cDNA synthesis.

Catalog Number	Mfr. No.	No. of Reactions
FERM1661	Thermo Scientific™ M1661	50 Reactions
FERM1662	Thermo Scientific™ M1662	200 Reactions

Description

Thermo Scientific Maxima H Minus Reverse Transcriptase (RT), an engineered RNase H minus enzyme, is formulated into convenient one-tube master mix with a simplified protocol, enabling consistency and maximum control of RT-qPCR. It was developed through molecular evolution to improve thermostability, robustness and synthesis rates. Maxima H Minus RT also shows up to a 50x increase in processivity, has no RNase H activity, and demonstrates higher sensitivity.

• Efficiently transcribes large and small amounts of RNA
• Quick and convenient setup eliminates pipeting caused variability
• Reduces risk of cross-contamination between samples
• Consistently higher sensitivity for wide variety of gene targets
• High linearity in cDNA synthesis over a broad dynamic range
• Complete enzyme activity from 42° to 55°C

Specifications

• Format: Master Mix
• Reaction Speed: 30 min.
• Optimal Reaction Temperature: 50°C
• Ribonuclease H Activity: Reduced
• For Use With (Application): Real Time PCR (qPCR), RT-PCR
• Final Product Type: First-Strand cDNA
• Reagent Type: Reverse Transcription
• Starting Material: RNA

• GC-Rich PCR Performance: High
• Technique: Reverse Transcription
• Reverse Transcriptase: Maxima H Minus
• Shipping Condition: Dry Ice
• Concentration: 5X
• Reaction Format: Premixed Components
• Size (Final Product): Up to 20 kb