Gibco™ Medium 199 (2X)

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells.

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco™ Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This Medium 199 is modified as follows:
With: L-glutamine
Without: HEPES, Phenol Red
The complete formulation is available.

Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO₂ incubator, or with Hanks' salts for use without CO₂. This Medium 199 is made with Earle's salts.

Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

Content And Storage

Storage conditions: 2 – 8°C. Protect from light.
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Cell Type

Mammalian Cells

Concentration

2 X

Culture Type

Mammalian Cell Culture

Form

Liquid

Product Type

Medium 199

Sterility

Sterile-filtered

With Additives

Glutamine, Earle's Balanced Salt Solution (EBSS)

Without Additives

No HEPES, No Phenol Red

Green Features

Sustainable packaging

Manufacturing Quality

cGMP-compliant under the ISO 13485 standard

Product Line

Gibco™

Quantity

250 mL

Shipping Condition

Room Temperature

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.