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Includes
The kit contains sufficient material for two 96-well plates.
The Click-iT RNA Alexa Fluor 488 HCS Assay enables detection of global RNA transcription temporally and spatially in cells (PNAS (2008) 105:15779-84). The ability to detect newly synthesized RNA or changes in RNA levels resulting from disease, environmental damage or drug treatments is an important aspect of toxicological profiling. Utilizing an alkyne-modified nucleoside, 5-ethynyl uridine (EU), and powerful click chemistry, newly synthesized RNA can be detected without the use of radioactivity or antibodies with a simple, two-step procedure. In step one, the alkyne-containing nucleoside is fed to cells or animals and actively incorporated into nascent RNA. The small size of the tag enables efficient incorporation of the modified nucleoside into RNA, but not into DNA. Detection utilizes the chemoselective ligation or “click” reaction between and azide and an alkyne where the modified RNA is detected with a corresponding azide-containing dye. With its dimunitive “footprint”, the Click-iT detection molecule can easily penetrate complex samples and leaves open the possibility of multiplex analyses with other probes, including antibodies for the detection of RNA-interactive proteins for deeper biological insights.
- Less hazardous
Cell Analysis, Cell Viability and Cytotoxicity, Cell Viability, Proliferation and Function, Cellular Imaging, Click Chemistries, DNA and RNA Purification and Analysis, High-Content Screening (HCS), Labeling Chemistry, Nascent RNA Detection and Capture with Click Chemistry, Nucleic Acid Labeling and Oligo Synthesis, RNA Labeling
Order Info
Shipping Condition: Room temperature
Specifications
Content And Storage
The kit contains sufficient material for 2 X 96-well plates. Store at 2-6°C, dessicate and protect from light.
Color
Green
Detection Method
Fluorescence
Format
96-well Plate
Label or Dye
Alexa Fluor 488
Excitation/Emission
495/519
For Use With (Equipment)
High Content Instrument
Green Features
Less hazardous
Product Line
Alexa Fluor™, Click-iT™, Molecular Probes™
Product Type
New RNA Synthesis Assay
For Research Use Only. Not for use in diagnostic procedures.
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