Invitrogen™ Dextran, Fluorescein, 70,000 MW, Anionic

Rigorous methods for removing as much unconjugated dye as practical, followed by an assay of dextran conjugates by thin-layer chromatography to help ensure the absence of low molecular weight contaminants

Labeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the hydrodynamic properties of the cytoplasmic matrix. The labeled dextran is commonly introduced into the cells via microinjection. Dextrans are hydrophilic polysaccharides characterized by their moderate-to-high molecular weight, good water solubility, and low toxicity. They also generally exhibit low immunogeniticy. Dextrans are biologically inert due to their uncommon poly-(α-D-1,6-glucose) linkages, which render them resistant to cleavage by most endogenous cellular glycosidases.

• Succinimidyl coupling of dyes to dextran molecule, which, in most cases, results in a neutral or anionic dextran
• Suitable for neuronal tracing (anterograde and retrograde) in live cells
• Cell lineage tracing in live cells
• Examining intercellular communications (e.g., in gap junctions, during wound healing, and during embryonic development)
• Investigating vascular permeability and blood-brain barrier integrity
• Monitoring acidification (some dextran-dye conjugates are pH-sensitive)
• Studying hydrodynamic properties of the cytoplasmic matrix

• The fluorescein dextrans are intrinsically anionic, whereas most of the dextrans labeled with the zwitterionic rhodamine B, tetramethylrhodamine, and Texas Red™ dyes are essentially neutral
• To produce more highly anionic dextrans, proprietary procedure adds negatively charged groups to the dextran carriers; these products are designated polyanionic dextrans

Cell Analysis, Cell Tracing and Tracking, Cell Viability, Proliferation and Function, General Cell Tracing, Ionic Homeostasis and Signaling, Neuronal Tracing, pH Detection

Order Info

Shipping Condition: Room temperature

Product Type

Dextran

Quantity

25 mg

Content And Storage

Store in freezer (-5 to -30°C) and protect from light.

Label or Dye

Classic Dyes

Shipping Condition

Room Temperature

Excitation/Emission

494/521 nm

Product Line

Invitrogen™

For Research Use Only. Not for use in diagnostic procedures.