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Invitrogen™ ImaGene Red™ C12RG lacZ Gene Expression Kit
The ImaGene Red™ lacZ Gene Expression Kit contains a fluorescein-based galactosidase substrate that has been covalently modified to include a 12-carbon lipophilic moiety, C12RG. Once inside the cell, the substrates are cleaved by β-galactosidase, producing a fluorescent product that is well retained by the cells, probably by incorporation of the lipophilic tail within the cellular membrane. In addition to the C12RG, the kits also include the broad-spectrum β-galactosidase inhibitor, PETG, and chloroquine diphosphate for inhibiting acidic hydrolysis of the substrate.
Beta-Galactosidase Assays, Cell Analysis, Cellular Imaging, Enzyme and Protein Activity Assays, Immunofluorescence (IF), Immunofluorescence Staining and Detection, Proteins, Expression, Isolation and Analysis, Reporter Assays
Order Info
Shipping Condition: Room Temperature
Specifications
Product Type
β-Galactosidase Reporter Gene Assay System
Detection Method
Fluorescence
Assay
β-Gal (lacZ) Assay
Content And Storage
Store in freezer (-5 to -30°C) and protect from light.
Shipping Condition
Room Temperature
Substrate
C12RG
Substrate Type
Beta-Gal Substrate
Substrate Properties
Chemical Substrate
Target Enzyme
Beta-Galactosidase
Format
Kit
For Research Use Only. Not for use in diagnostic procedures.
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